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The F-12 medium containing low serum (1% HS and 0.5% FBS) was replaced prior to exposure to vehicle (0.1% DMSO) or indicated reagents.
Human RBC have been stored successfully at −80 °C in a medium containing low concentrations of synthetic, small molecule IRI and at a low glycerol concentration (15%)44,45.
More cellulase was produced in the sludge medium containing low concentrations of Ni2+, Zn2+ and Cu2+ ions.
When the CEN1 HC-Br derived peptides were evaluated for anti-bacterial activity against S. aureus and P. aeruginosa, all peptides showed pronounced microbicidal effect in assay medium containing low salt concentrations and no serum (0.037% BHI).
Post-germination growth was slightly reduced when the two OsNCED4-overexpressing lines were grown on medium containing low amounts of Glc, but it was severely inhibited under high Glc condition (Fig. 4a, b).
Cells grown in medium containing low (170 mM) and high (620 mM) initial glycerol concentrations were harvested for the proteomic analyses described below from exponential and stationary phase cultures.
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PSM; is a basal medium containing low-glucose Dulbecco's Modified Eagle Medium (Cambrex BioScience), 100 U/ml penicillin/0.1 mg/ml streptomycin (Invitrogen), and 2 mM Ultraglutamine (Cambrex BioScience) with or without 10 μM of S1P (Biomol, Exeter, UK) and 20 ng/ml PDGF (Peprotech, NJ, USA) as described in Pebay et al., 2007.
NIM-1 and K1 conditioned media contained 50 and 150 pg/106 cells of TGFA, respectively, and TPC1 medium contained low but evaluable amount of TGFA.
The culture medium contained low glucose DMEM (Hyclone, Logan, Utah, USA) supplemented with 10% fetal bovine serum (FBS) (Hyclone), 100 IU/ml penicillin (Hyclone), and 100 µg/ml streptomycin (Hyclone).
In accordance with the findings in fibroblasts, migration of MDA-MB-231 cells was substantially higher when the medium contained low concentration (3 μM) of ML-7 as compared to diluent alone.
Both yeast strains were cultured in the nutrient-rich YP (1% yeast extract and 2% peptone) medium initially containing low (0.2%) concentration of glucose, a fermentable carbon source.
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