Sentence examples for medium containing leucine from inspiring English sources

Transformants were grown on minimal medium containing leucine only (MM/L).

Furthermore, deletion of arn1+ in a tsc2Δ background suppressed a growth defect of a leucine-requiring tsc2 mutant on EMM medium containing leucine (Fig. 2B).

H. polymorpha DL-1 was grown up to OD660 ~2.0 in 0.67% YNB medium containing leucine (20 mg/l) and either 1% glucose or 1% methanol at 37°C while shaking at 250 rpm.

Liquid pre-cultures of minimal medium containing leucine (0.24 g/l), methionine (0.115 g/l) and uracil (0.05 g/l) were inoculated from the minimal medium plates with 10 g/l glucose.

It has been known that a tsc2 deletion mutant is resistant to a high concentration of canavanine and shows a growth defect in the minimal medium containing leucine when the mutant has a leucine auxotrophy, owing to the defect of amino acid uptake (Matsumoto et al., 2002; van Slegtenhorst et al., 2004).

The transformants (two independent colonies for each transformation) were streaked on growth plates (minimum synthetic dropout (SD) medium containing 2% glucose and 50 µg/ml leucine) or on selection plates (SD medium containing 1% galactose, 0.2% raffinose, BU salts, and 80 µg/ml X-gal).

Correct integration was verified by PCR.> Strain BY4741 was grown overnight at 30°C in Translucent YNB medium containing 50 mM KCl, supplemented with leucine, histidine, methionine and uracil.

The incubation was continued for 1.5 h (Stx) or 2 h (ricin), then the medium was replaced with leucine-free Hepes medium containing 2 µCi/ml [3H]leucine, and the cells were further incubated for 20 min. The proteins were precipitated with 5% TCA, washed once in 5% TCA, and then dissolved in 0.1 M KOH.

At the end of the labeling period, cells were washed five times with PBS and incubated with chase medium containing 50 times the molar concentration of leucine for two h to chase-out short-lived proteins.

All seven transformants were chosen based on their robust growth on medium lacking leucine and tyrosine and on medium containing canavanine prior to and after cre-induced recombination, respectively.

Transformants containing gap-repaired plasmids were selected on SC medium lacking leucine and then replica-plated onto medium containing 5-FOA to select for cells that lost the URA3 -marked plasmid carrying a wild-type copy of SPT16 (p SPT16 - URA3 ).