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The functionality of these chimeras was assayed in growth tests in medium containing lactate as sole carbon source.
Mansour et al. (2008) studied the growth of a strain of Y. lipolytica isolated from cheese in liquid medium containing lactate in the presence of high or low concentrations of amino acids.
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Spermatozoal motility was analyzed, intracellular calcium was measured, and the ability of sperm to undergo acrosome reaction was compared after incubation in a skim milk extender (SME) and Tyrode's medium containing albumin, lactate, and pyruvate (TALP) at 37 °C.
The robust growth of the Detrick strains relative to 1942 in low-pH medium containing high lactate levels is likely due to mutations in mmgD (2-methylcitrate synthase, position 2029530), or a short-chain 3-oxoacyl-[acyl-carrier-protein] reductase (position 3437350), or both.
DvH was grown in LS4D medium, which contains lactate as the carbon source and electron donor and sulfate as the electron acceptor (modified to 2.5 μM resazurin and 130 μM riboflavin in modified Thauer's vitamins stock).
Solutions containing lactate were found to yield spherical calcite crystals.
Lactate/lactic acid has been considered as a biofuel for enzymatic biofuel cells, but only with single enzyme bioanodes containing lactate dehydrogenase.
All of the engineered R. eutropha strains produced PHAs containing 2-hydroxyacid monomers, including lactate and 2-hydroxybutyrate (2HB), along with 3-hydroxybutyrate (3HB) and/or 3-hydroxyvalerate (3HV), when they were cultured in nitrogen-free medium containing 5 g/L lactate or 4 g/L 2HB and 20 g/L glucose as carbon sources.
This was most clearly illustrated by the sensitivities of cells grown in medium containing glucose plus lactate.
Noteworthy, color of the medium containing 30 mM lactate was similar to yellow color of CM in the stationary culture, when cells undergo CS.
Transferring and adapting the strains several times to the medium containing perchlorate and lactate or methanol did not result in a substantial reduction of perchlorate to chloride.
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