Sentence examples for medium containing insulin from inspiring English sources

Here, we evaluated the effects of a new transfer medium containing insulin and β-fibroblast growth factor, which can increase the viability of fat grafts before performing autologous fat transplantation.

Autologous fat transplantation using the new transfer medium containing insulin and β-fibroblast growth factor is a simple, promising strategy for clinical application designed to augment the viability of fat transplantation.

Cardiac myocytes were cultured in DMEM/F-12 medium supplemented with 5% fetal bovine serum and 100 µmol/l 5-bromo-2-deoxyuridine (BrdU) for 16-24 hours, and culture medium was then changed to serum-deprived medium containing insulin, transferrin, selenium, bovine serum albumin and BrdU.

After 2 days, the medium was replaced with fresh complete medium containing insulin, and the cells were incubated for an additional 2-day period.

For experiments in which cells were seeded in medium containing insulin, cells were washed with HBSS to remove insulin, before administration of hormonal treatment.

This was followed by a further two days in growth medium containing insulin (1  μg ml−1), and growth medium was then changed daily.

The culture medium contains insulin and the mitogen basic Fibroblast Growth Factor (FGF2).

This biochemically defined medium contained insulin (5 ng/ml), Progesterone (20 nM), Putrescine (100 μM), Selenium (5 ng/ml), apo-Transferrin (50 μg/ml) and penicillin/streptomycin (50 ung/ml50 ng/ml).

After 2 days in culture, the growth medium was replaced by a low-insulin medium [DMEM/F12 medium supplemented with an alternative F27 medium containing no insulin (Invitrogen) and with 5 ng/ml of insulin].

After 8 days in differentiation medium, containing 85nM insulin, 2nM triiodthyronine (T3) and 10% FCS, cells were stained with OilRedO (ThermoScientific) [34].

After two days this was replaced with medium containing 5μg/ml insulin and FCS.