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Growth comparisons between XR122N and AJP50 in a model hydrolysate medium containing inhibitory compounds found in pretreated biomass showed that AJP50 exited lag phase faster under all conditions tested.
For each strain, the tolerance was evaluated as relative growth (optical density (OD) value, %) by comparing the yeast growth in the medium containing inhibitory compound(s) with that in medium lacking these compound(s).
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The ESCs selectively attached to the micropatterned features and expressed markers associated with a pluripotent phenotype, such as E-cadherin and alkaline phosphatase, when maintained in growth medium containing leukemia inhibitory factor.
For culture, embryos were then cultured in complete ES cell medium containing leukemia inhibitory factor.
Briefly, ES cells were kept in standard ES cell medium containing leukemia inhibitory factor (LIF) which prevents cell differentiation.
They are typically cultured on feeders of mitotically inactivated fibroblasts in medium containing leukemia inhibitory factor (LIF), which is required for maintenance of a pluripotent state capable of generating any cell type in the body [ 23].
The bacteriostatic versus bactericidal nature of the extracts was determined by subculture from wells containing inhibitory dilutions into neat SP-4 or mT-soy medium (broth and agar) at 37°C.
In fact, when the sod2Δ mutant cells were grown for 24 h in YPD medium containing a sub-inhibitory concentration of CTBT the respiration deficient petite mutants were induced in high frequency indicating the damage to mtDNA induced by CTBT (Table 5).
For condition II the cells were cultured in gelatine-coated dishes with basic medium containing 103 units/ml leukaemia inhibitory factor (LIF; ESGRO, Millipore, Billerica, MA, USA).
The CD45 + splenocytes were then treated with acidified HBSS (pH 5.7) for 25 min at 37°C and maintained in DMEM/F-12 culture medium containing B27 and Leukemia Inhibitory Factor (LIF) for one to seven days.
Cells were cultured in 96-well plates in 200 μl of medium containing 2.5% serum and the inhibitory compounds.
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