Sentence examples for medium containing increasing from inspiring English sources

Fresh medium containing increasing three different concentrations of the GNPs (10, 50, and 100 μl) was added to each well and the cells were incubated for 6, 24, 48, and 72 h time periods.

A mixed population of cells that grew exponentially in the present of 15 nM of BPR0L075 were obtained and further cultured in complete medium containing increasing concentrations of the drug to 20 nM of BPR0L075.

Then, cells that grew exponentially in the present of 20 nM of BPR0L075 were obtained and subsequently cultured in complete medium containing increasing concentrations of the drug to 30 nM of BPR0L075.

After overnight growth at 37°C under rotating agitation, the bacterial suspension was used to initiate cultures in 10 ml of SS medium containing increasing concentrations of nicotinate or MgSO4.

Briefly, KB cells were initially incubated in completed medium containing 5 nM of BPR0L075 that yielded 40% cell survival for a period of 3∼4 weeks, and the cells that proliferated were repeatedly subcultured in completed medium containing increasing concentrations of the drug (at 20% increment each time).

After digestion, the cells were resuspended in modified Eagle's medium containing increasing Ca2+ concentrations (200 μM, 500 μM, and 1 mM).

Specifically, we examined the membrane traffic mutants from all three classes identified in the original screen by asking whether growth on medium containing increased KCl suppressed the carboxypeptidase Y (CPY) secretion phenotype exhibited by these strains.

A 10% (v v−1) inoculum from a previous culture was sequentially transferred to the fresh medium containing increased concentrations of lignin at 3.2, 3.4, 3.6, and 3.8 g L−1 after 3, 6, 10, and 15 days of cultivation.

N2a neuroblastoma cells were incubated in serum-supplemented or serum-free media and the serum-free medium contained increasing concentrations of CysC.

To determine whether or not each of the mutants could support the propagation of these aggregates following the depletion of New1 and wild-type ClpB, we plated the starter culture cells at the nonpermissive temperature on solid medium lacking or containing increasing concentrations of aTc, generating sets of R1 colonies.

To test the cellular resistance to MMC, clonogenic assays were conducted in semisolid medium (Methocult H4434, StemCell Technologies) containing increasing concentrations of the drug, plated in triplicate on 35-mm plastic culture dishes (Nunc, Roskilde, Denmark) and cultured at 37°C, 5% CO2 and fully humidified air.