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Adk−/− or wild type (wt) ESC-derived glial precursor cells were seeded on the substrates and cultured either in proliferation medium containing growth factors or in differentiation medium devoid of growth factors.
The lower well contained 600 µl of 0.5% FBS McCoy's medium containing growth factor (EGF, 20 ng/ml).
The cells were seeded in 96-well plates at 10,000 per well in EBM2 medium containing growth supplements (bullet kit; Lonza) and 10% FBS.
For StdD differentiation, NSCs were seeded at 30,000 cells/cm on laminin and expanded to 80% confluency in B27 medium containing growth factors (+GFs).
Half of the medium was replaced with fresh medium containing growth factors on day four and every two or three days after that.
The cells were then released from starvation with DMEM/F12 medium containing growth factors and serum.
Similar(34)
Twenty-four hours after infection, medium was changed into the selection medium (1.5 µg/ml puromycin containing growth medium).
The bottom chamber was filled with complete growth medium containing chemoattractant growth factors.
Growth effects of E2, G1 and Tam were investigated in phenol-red free medium containing sufficient growth factors to support growth of cells.
A commercially available SMC medium (Cascade Biologics) containing growth and differentiation factor supplements was used.
For example, culture conditions conducive for chondrogenic induction of MSCs require high-density pelleting and growth in serum-free medium containing specific growth factors and supplements.
More suggestions(13)
medium containing galactose
medium containing ampicillin
medium containing phytohemagglutinin
medium supporting growth
medium showing growth
medium containing fenretinide
medium containing chemical
medium containing dye
medium containing ethanol
medium containing glycerol
medium containing control
medium containing chloramphenicol
medium containing agar
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