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The second was samples obtained from liquid culture medium containing growing mycobacteria (BACTEC, Becton Dickinson).
We saw no significant alteration in the levels of procaspase-3 or the active p20 and p11 caspase 3 fragments in CD4+ T-cells incubated for 20 h in the presence of medium containing growing concentrations of TSA.
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Colonies were placed in 150 μl liquid LB medium containing ampicillin, grown overnight at 37°C, and stored at -80°C after adding 20 μl of sterile glycerol.
In addition, the spirochete is only able to grow in medium containing lipids, and grows well when cholesterol and phophatidylcholine are included [34].
Isolated colonies were used to prepare primary inoculum by inoculating in 5 ml LB medium containing antibiotics and growing overnight aerobically.
Three colonies were inoculated into fresh LB medium containing Amp100 and grown overnight.
A single colony was inoculated to 10-mL LB medium containing antibiotics and grown overnight at 37°C.
50 μl of this preculture was transferred to 50 ml of LB medium containing kanamycin and grown at 37°C.
Transformed cells were inoculated at a dilution of 1 100 in each 1000 ml fresh LB medium containing ampicillin and grown aerobically at 37 °C.
The growing medium containing mycelium and spores was dissolved in extraction buffer (0.82 mM sodium citrate and 0.18 mM citric acid).
Seeds were prepared as described above and sown onto sterile filter paper in contact with growing medium containing 1% (w/v) sucrose.
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