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They were maintained in Roswell Park Memorial Institute 1640 (RPMI 1640) medium containing glutamine (2 mM) and foetal calf serum (10%).
As evidence for our hypothesis, P. purpurogenum IAM15392 was transferred to fresh basal medium after it was cultured in medium containing glutamine and then was grown until the cultivation time reached a total of 96 h.
COS-1 cells were cultured in high glucose Dulbecco's modified Eagle's medium containing glutamine, penicillin, streptomycin, and 10% FBS.
Spodoptera frugiperda (Sf9) cells were grown at 27°C in serum-free Bio-insect medium containing glutamine, penicillin, streptomycin and amphotericin (Biological Industries, Israel).
African Green monkey CV-1 cells (ATCC # CCL-70), Human Embryonic Kidney 293 cells (ATCC #CRL-1573) and Mouse Tubular cells [21] were cultured in high glucose Dulbecco's modified Eagle's medium containing glutamine, penicillin, streptomycin, and 10% FBS.
Both cell lines were cultured in DMEM medium containing glutamine (2 mM), antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin), and 10% FBS in a humidified atmosphere at 37°C and 5% CO2.
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The culture medium consisted of ATCC medium (DMEM/F-12 Ham containing glutamine and sodium pyruvate; Sigma) supplemented with 10% fetal bovine serum (Sigma).
Cells were cultured in medium containing [1-13C] glutomine to assess the level of reductive glutamine metabolism via the reverse reaction of isocitrate dehydrogenase (IDH).
It was experimentally induced by depriving cells of glucose in a medium containing no glutamine, pyruvate, lactate or serum, and an equal amount of viable TCs were systematically compared.
The cells propagated in medium containing no glutamine and glucose also demonstrated the presence of some rounded shrunken cells and decreased cell density.
Normal human dermal fibroblasts (NHDFs) were purchased from Lonza and cultured in DMEM/F12 medium containing L-glutamine and pyruvate (Invitrogen) and supplemented with 10% FBS (Promega).
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