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L5178Y cells were grown in medium containing from 0 to 80 μmol/l arginine for 24 hours then transferred to fresh medium for 24 hours.
To analyze the effect of bFGF on proliferation and cell morphology, TMSCs were cultured in medium containing from 0 to 50 ng/ml bFGF.
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Accumulation of TNFα protein in conditioned medium containing PBMCs from DLE patients, but not from SCLE, TLE or DM patients, was significantly greater (19-fold) than that from controls (P < 0.001).
Cells were then treated with fresh medium containing SF from RA patients, or TNF.
MSCs were cultivated for 5 days in expansion medium containing serum from aged and young rats, respectively.
Forty-eight hour-conditioned medium (containing serum) from SCC4 cells diluted 50% in Dulbecco's modified Eagle's medium was added to cultures of human PBMCs.
The BI-S-33 medium containing Fe3+ (from AFC) or low iron medium supplemented with B-holo-Lf was tested in order to determine the iron quantity.
On day 2 cells were harvested, washed and then exposed to fresh medium containing SF from RA patients diluted to 1 5 or 1 10.
Briefly, exponentially growing cells were seeded into 96-well plates and were incubated in medium containing letrozole from 7 nM to 0.7 μM for 48 hours at 37°C.
To address this issue, we collected cell-free medium containing SEAP from LS8-SEAP cells grown in the absence of F−.
Similar experiments with incubation medium containing SF from patients with OA revealed that 3.9% ± 0.5% of neutrophils expressed membrane RANK-L (n = 14).
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