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Culture medium containing floating cells was removed and retained.
Medium containing floating cells was gently removed and cells were observed using fluorescence microscopy (Olympus).
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The medium, which contained floating cells, was exchanged for fresh medium at Day 3. At 10 days of culture, adherent cells with a spindle-like morphology were counted under an inverted microscope.
The medium containing the floating OPC was replated on to poly-lysine-coated Petri dishes (Falcon).
Floating aquatic plant systems usually contain floating macrophytes.
Do not use water that contains floating material, has an odor or is dark in color.
We seeded bone marrow cells on plastic dishes in medium containing serum, removed floating cells four days thereafter, and added PDGF-BB to the adherent cells.
Adult NSCs and progenitors can be grown in proliferation medium containing EGF and FGF2 as floating colonies of cells or neurospheres [56], [66].
Monodispersed polystyrene microspheres were self-assembled by floating in the medium containing ethylene glycol, dried, annealed and infiltrated with heparin/chitin/chitosan gels.
Negative control peels were floated on MS medium containing an appropriate volume of the drug solvent only.
The other approach we called 'bath', because skin was floated on complete medium containing 5 μg/ml targeting mAb for 24 h to allow the antibody to diffuse into the tissue.
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