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S. solfataricus P2 was grown at 75 °C in Brock's medium containing either KH2PO4 to a final concentration of 280 mg/l (+P) or shifted to Brocks medium without KH2PO4 (−P; phosphate-limiting conditions).
Y. lipolytica JMY1212 wide type and recombinant strains overexpressing BGL1 and BGL2 were grown to mid-exponential phase in defined media and then transferred into fresh medium containing either glucose or cellobiose as the sole carbon source.
Infected explants were placed on shoot induction medium containing either 5 mg L−1 hygromycin or 75 mg L−1 kanamycin for selection.
At each time point, the medium was replaced with fresh medium containing either 45 μM (low glucose) or 180 μM (high glucose) 2-NBDG, and was incubated at 37 °C for 45 minutes.
The medium was replaced with medium containing either nanoparticles or free Nile Red, or the dual-labelled nanoparticles.
Similar to TMB4443, TMB4425 was also characterized in a medium containing either glucose or xylose and under aerobic or anaerobic conditions.
Even at a concentration as low as 1 μM, CF inhibited the growth of strain DCB-2 in medium containing either 3-Cl-4-OHPA or fumarate.
After cells had stabilized, fresh medium containing either Z CFX, ZLH or CFX at different concentrations (0.5, 5.0, 50.0 and 500.0 μg/mL) was added and incubation continued for 72 h.
After 24 hours, the medium was changed to challenge medium containing either DMSO or compounds.
The following day the cells were incubated with fresh medium containing either 10 µM LY294002 or DMSO (equal volume) for an additional 24 hours.
H2Kd+HLA+ cells were grown in CFU medium containing either mouse recombinant growth factors or human recombinant growth factors for 18 days.
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