Twenty-four hours later, medium was exchanged against 100 μl of medium containing different concentrations of verteporfin (0.001 10 μM) was added.
To verify the Gal-3 effect on the cells, we performed growth curves in Sabouraud liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar, NY, USA) using an iEMS spectrophotometer (Thermofisher).
Promoters strength was initially roughly assessed by patch replica plating on HT medium containing different concentrations of neomycin.
A549 cells were cultured in a medium containing different concentrations of the four extracts for 48 h.
The differentiated adipocytes were replaced the medium with 100 μL fresh growth medium containing different concentrations of test samples.
aCells were cultivated for 48 h, at 30 °C, 200 rpm in MM medium containing different concentrations of 4HB-related carbon sources at two-stage cultivation.
The chemostat cultivation was operated at different dilution rates ranging from 0.05 h-1 to 0.2 h-1 with the feed medium containing different carbon sources.
After 24 h incubation, cells were washed with PBS and exposed to fresh culture medium containing different DNA sequences and then washed by PBS.
The cells were washed with phosphate buffered saline (PBS, pH 7.4) and incubated in fresh medium containing different concentrations of AuNPs dissolved in water.
After 24 h, the medium was removed and replaced with 100 μl medium containing different concentrations of MSPs (10 and 25 μg/ml).
Toxicity test is determined by the cell viability after incubation in a culture medium containing different concentrations of CdSe/ZnS QDs.
