After day 32, medium containing chemicals was replaced with the 2i-medium.
If the nutritional requirements of a microorganism are known, a chemically defined medium containing only those chemicals can be prepared.
The medium was removed and replaced by fresh medium containing the test chemicals.
Then, the medium was removed and fresh medium containing the test chemicals was added.
The durability can be described as concrete's resistance to the destructive influences of a medium containing acid, sulfate and/or various chemicals and mechanical effects.
hESC-derived neural crest cells were exposed to chemicals in N2 medium containing EGF (20 ng/ml) and FGF2 (20 ng/ml).
Ferreum got him going with the medium, and soon after it he created Ferroux, this time mixing tiny portions of ferrofluid with other liquids containing chemicals—soap, alcohol, milk, etc.
FBS-free medium was then replaced by a medium containing 10% DCC-FBS, hormones, and test chemicals (or vehicles) for a 24-hour incubation period at 37°C.
Radio-opaque contrast medium containing lead oxide (P3O4 Red lead, AJAX Chemicals, Australia) was injected antegrade with a micromanipulator (UM-3C, Narishige Co., Japan).
Then a medium containing 10% DCC-FBS, the hormones, and test chemicals (or vehicles) were added.
