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Reaction medium was prepared by adding synthetic indigo and the water extract of Bokbunja sludge in a medium containing calcium hydroxide (5 g/150 ml).
In brief, hearts excised from anesthetized mice were subjected to Langendorff perfusion with Krebs medium containing calcium (2.5 mM) for 5 minutes, followed by perfusion with Calcium free Krebs medium (8 10 minutes).
As a consequence of precipitation of monobasic potassium phosphate with calcium, it was not included in medium containing calcium.
EA uptake by HeLa cells in calcium-free media was significantly reduced compared with medium containing calcium (Fig. 5A).
The following day, normal growth medium (containing calcium) was added to allow the re-formation of TJs and restoration of cell cell contacts.
Fully polarized MDCKII cell monolayers were switched from a medium containing calcium to a medium devoid of calcium to induce junctional complex disassembly (Ivanov et al, 2004 and see Section 4).
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The cultured cells were washed twice (1 mL medium/well), and then incubated with new cell culture medium (1 mL/well) containing calcium binding reagent, Fluo-8 (50 μg).
Assuming this calcium-dependent increase in cell death is not cell-type specific, one would expect HeLa to have a lower LD50 than Jurkat; however the opposite is presented in Figure 2. Furthermore, one could expect that if HeLa were placed in medium containing less calcium, they may be even more resistant to USEP-induced death.
These receptors were activated in a medium containing extracellular calcium.
In order determine whether TACSTD2 is required for keratinocyte differentiation, siRNAs to silence Tacstd2 expression was used to transfect HaCaT cells cultured in medium containing different calcium concentration, and the expression of genes associated with keratinocyte differentiation was analyzed.
After treatment for 21 days with osteogenic medium, the pMSCs were embedded in mineralizing matrix containing calcium.
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medium containing serum
medium containing polymer
medium containing glucose
medium containing galactose
medium containing ampicillin
medium raising calcium
medium containing dye
medium lacking calcium
medium containing ethanol
medium containing glycerol
medium containing test
medium containing control
medium containing chloramphenicol
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