MSCs in 96-well plates (5,000 cells per well) were incubated with MSC medium containing 50 μg/mL of amino-modified FMNPs for 4 h at 37 °C.
The RPE cell line ARPE-19 obtained from American Type Culture Collection (Masnassas, VA) and RGC5 cells [32] were grown in complete medium containing Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA), 10% fetal bovine serum, essential and nonessential amino acids, and antibiotics.
The human embryonic stem cell line, H1 (WiCell, Madison, WI, USA), was grown on irradiated mouse embryonic fibroblasts in medium containing Dulbecco's modified Eagle medium (DMEM):F12, serum replacer (Invitrogen, Paisley, UK), l-glutamine, β-mercaptoethanol, non-essential amino acids and basic fibroblast growth factor (8 ng/ml).
The control gingival fibroblast was cultured in a medium containing Dulbecco's modified Eagle medium for 3 months.
The experimental group 1 gingival fibroblast was cultured in a medium containing Dulbecco's modified Eagle medium for a first stage, and cultured in a medium containing Minimum essential medium for a second stage.
HDFs and Plat-A cells were maintained in medium containing Dulbecco's modified Eagle's medium (DMEM; Gibco, 11965-092), 10% fetal bovine serum (FBS, Thermo, Shanghai, China), 1% nonessential amino acids (NEAA, Gibco, 11140-050) and 1% GlutaMAX (Gibco, 35050).
Harvested nasal cartilage discs was maintained by culture in basic medium containing Dulbecco's modified Eagle's medium (DMEM, Sigma) with 10 m m Hepes buffer (Sigma), 1.0% (v/v) P/S, 1.0% (v/v) non-essential amino acids (NEAA; Sigma), Glutamax (Sigma), and 10% amphotericin B (Sigma) at 37 °C in a 5% CO2 environment.
J774 cells were propagated in RPMI or Dulbecco's Modified Eagle's Medium (DMEM) medium containing 10% fetal calf serum.
Proliferating C2C12 myoblasts were kept in GM (growth medium) containing DMEM (Dulbecco's modified Eagle's medium) supplemented with 20% (v/v) FCS (fetal calf serum).
MCF-7 cells were obtained from the European Collection of Animal Cell Cultures (ECACC No. 86012803; CB No. CB 2705), and their culture medium was composed of Dulbecco's modified Eagle medium, containing 1% nonessential amino acids and 10% foetal calf serum.
