Your English writing platform
Discover LudwigSuggestions(2)
Exact(5)
Agarase production by this bacterium has been proven by the appearance of depressions around bacterial colonies when cultured in solid culture medium containing agar.
The dilutions (1 mL) were mixed with 15 20 mL BCP medium containing agar (Nissui Pharmaceutical Co., Ltd.; Tokyo, Japan), and the plates were incubated at 25°C for 72 h.
Moreover, this conclusion was further supported by covering infected DBCC with medium containing agar, limiting free movement of released infectious particles.
The cell suspension (1 × 10 cells) were mixed with equal volume of growth medium containing agar (0.6%) so that final concentration of agar becomes 0.3%, which was overlaid onto the bottom layer.
In order to assess possible contamination and obtain estimates of final growth densities, following the transfer to new medium dilutions were plated onto solid medium containing agar (1.8% w/v, Difco) supplemented with 125 mM methanol to permit larger colonies to form.
Similar(55)
The cells were then gently mixed with 7 mL of top agar (synthetic complete medium containing 2.5% agar, 1M sorbitol and required aminoacids except adenine and uracil) kept at 45°C.
Cells were harvested and mixed (500, 1000, 1500 cells per well) with tissue culture medium containing 0.7% agar to a final agar concentration of 0.35%.
Briefly, cells were seeded in 24 well-plates at a density of 200 cells/well into culture medium containing 0.3% agar (Roth, Karlsruhe) overlaying 0.5% agar.
At 2 days after transfection, MCF-7 cells (300 cells per well) transfected with indicated plasmids were mixed with tissue culture medium containing 0.7% agar to result in a final agar concentration of 0.35%.
Cells were suspended in medium containing 0.3% agar and plated onto a layer of 0.6% agar (for each well, 4000 cell in 800 μl medium, 1 ml bottom agar).
Cells were re-suspended at 5×10 cells ml−1 in medium containing 0.3% agar and 2 ml layered on top of the base agar.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com