Subsequently, different stresses were added into the PDB medium to make the PDB medium contained the 50 μM CdCl2, 1% NaCl or 2 mM H2O2 respectively.
Semi-selective medium contained the following: 37.5 mg carbendazim, 37.5 mg thiabendazole, 75 mg rose bengal, 17.5 mg NaCl, 50 mg each of streptomycin sulphate, chlortetracycline hydrochloride and chloramphenicol, 3 ml Triton X-100, and 17 g corn meal agar (Difco) in a liter of distilled water.
For all cultivations, the nitrogen-limited medium contained the following components per liter: 2 g Na2HPO4⋅12H2O, 14.9 g KCl, 46.72 g NaCl, 14.5 g Tris, 2.05 g MgCl2, 3.53 g Na2SO4, 1 g (NH4 2 SO4, 1 g MgSO4⋅7H2O, and 2.5 mL of trace element solution.
The culture medium contained the final concentration of DMSO at 0.6%, when cells were treated with the reagents as indicated in each experiments.
High glucose medium contained the same fermentor media (with cells removed), but with glucose added back to a concentration of 1% (w/v).
In turn, the group-beneficial QS-selective medium contained the protein BSA as the main carbon source.
Transformants, initially selected in G418-containing medium, were then able to grow in medium containing the NBP alendronate (160 μM).
After selection, the medium containing the yeast and newspaper was directly transferred to SD+M medium plate.
A non-physiologically relevant medium containing the cationic surfactant, cetrimide, was also investigated.
The medium containing the secreted protein was collected after 3 4 days of incubation.
Finally, the aqueous medium contains the calcium (Ca2+) and carbonate (CO3 2−) ions.
