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In this experiment, the culture medium contained a glucose and cellobiose mixture with respective initial concentrations of 47 and 43.3 mM.
The additional medium contained a hydrolyzed sucrose solution as the main carbon and energy source and it was added after the glucose depletion.
The average shoot length was higher when the medium contained a low BA concentration (0.1 mg/l) in combination with IAA, NAA, or 2iP. Figure 1 Callus and adventitious shoot regeneration of leaf explants of Hygrophila pogonocalyx cultured with different concentrations (mg/l) of plant growth regulators.
The differentiation medium contained a 1∶1 mixture of DMEM and small airway epithelial growth medium supplemented as described above, except that gentamycin sulfate, amphotericin B, and triiodothyronine were replaced with 1% penicillin/streptomycin, and 50 nM all-trans retinoic acid.
The T84 culture medium contained a 1∶1 mixture of Dulbecco-Vogt modified Eagle medium and Ham's-F12 medium (DMEM/F12) supplemented with 50 µg ml−1 penicillin, 50 µg ml−1 streptomycin (Sigma, France), and 4% fetal bovine serum (Hyclone, France).
This medium contained a high concentration of Fe2+ and no Mn2+.
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The final growth medium contained an inoculum concentration of 1.0 × 105 cfu/ml (colony forming units).
For the UCSD-AML1 cell line, the used medium contained an additional 10 ng/ml of GM-CSF (Promocell, UK).
Nuclear photographic emulsion, also called Nuclear Emulsion, radiation detector generally in the form of a glass plate thinly coated with a transparent medium containing a silver halide compound.
Five enteric bacterial populations were evolved by point mutation in medium containing a single limiting resource.
The problem is equivalent to a two-dimensional 'quasi-plane' piezoelectric medium containing a 2D inclusion.
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