The isolated bone marrow cells were seeded for expansion in complete isolation medium constituted of RPMI-1640 (GInvitrogentrogen, Carlsbad, Ca, USA) supplemented with 20% fetal bovine serum (Invitrogen), 100 units/ml penicillin and 100 µg/ml streptomycin (Invitrogen).
Live sperm suspensions (10x106 sperm/mL) were incubated in a PBS-Glucose-BSA (PBS-Glu-BSA) medium (an appropriate sperm culture medium constituted of 1 g/L of glucose and 3 g/L of BSA) with SYBR-14 (100 nM) and PI (240 nM) for 10 minutes, at 37°C, in the dark.
In optimization experiments, however, use was made of larger vessels (100-mL bottles) and the medium constituted not more than 20% of the total volume.
Myc11 was grown in minimal medium constituted with [N]NH4Cl.
Indicator medium The use of indicator medium constitutes a direct way to detect particular small molecules, chemical reactions, or metabolic, catabolic, or antibiotic capabilities of a clone.
In Brazil about 73% of the software industry is constituted of small and medium-sized enterprises (SMEs) (MCTI [2013]).
The negative control, which constituted of the same culture medium used for viral inoculation but without the virus, was seeded in another six wells.
Now, the higher the carrier, the more the signal is attenuated by the medium, which constitutes one of the fundamental tradeoffs in radar systems, namely, Cross-range Resolution × Depth of penetration.
It is well known that most losses from natural hazards result from the cumulative effects of high-frequency, low-impact disasters; the poorest members of the community, low income households, and small and medium enterprises constitute a high percentage of all losses (UNISDR 2013; Co-Chairs of the Preparatory Committee 2014).
For the statistics, small, medium, and large constituted one group of 250 smaller ICA aneurysms.
Just as the electromagnetic fields are not states of a medium but constitute independent realities which are not reducible to anything else, so, according to Weyl, the geometrical fields are independent irreducible physical fields.[62] A class of geometric structural fields of a given type is characterized by a particular Lie group.
