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Central composite design was applied to optimize the medium constituents and explain the combined effects of three medium constituents, viz., sucrose, yeast extract and potassium nitrate.
Concentrations of other medium constituents were at the same level as previously.
Other medium constituents include whey permeate (20 g/L) and urea (2 g/L).
Response surface methodology (RSM) was applied to optimize the medium constituents.
A 22 full-factorial central composite design was chosen to explain four medium constituents, viz.
Medium constituents were optimised for the production of lipase by central composite design.
Statistical tools were employed to optimize the medium constituents to enhance lipase-yield.
The variables A H represented the medium constituents and I K represented the dummy variables/unassigned variables.
An un-inoculated control reactor was run to account for background VOCs resulting from the medium constituents or air supply.
By varying the medium constituents we could control the size and shape distributions of the gold nanoparticles.
Subsequently, a Box Behnken design was used for outlining the concentration of the most effective medium constituents.
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