After the second wash, the cells were resuspended in induction medium consisting of BMMY or BMGY with β-estradiol.
Soil is considered as a three-phase medium consisting of solid particles, water and air.
A one-dimensional medium consisting of two alternating states is first studied.
The cells were confluent the next day and the medium was changed to medium consisting of 3% FBS, 0.1 mM NEAA, and 2% DMSO.
In a medium consisting of the projection of impressions on a light-sensitive material, lighting is of special importance.
The complex medium consisting of sucrose, peptone and yeast extract was found to be the best composition for PIP production.
The lithophysal rock is described as a tri-continuum medium, consisting of fractures, rock matrices, and cavities.
Cells were cultured in standard medium consisting of low-glucose DMEM (Life technology, USA) supplemented with 10% FCS (Biochrom, USA) at 37 °C and 5% CO2 in a humidified incubator.
The chondrogenic cell line ATDC5 (Riken BioResource Center, Tsukuba, Japan) was cultured in maintenance medium consisting of Dulbecco's modified Eagle's medium (DMEM) nutrient mixture F12 (DMEM:F12) (Gibco) supplemented with 5% fetal bovine serum (FBS) (Gibco).
Colonies of hESC were dispersed by dispase/mechanical dissociation and transferred into EB differentiation medium, consisting of DMEM/F12, 15% FBS, 1% non-essential amino acids, 1 mM L-glutamine, and 0.1 mM 2-mercaptoethanol, in low attachment plates (Corning).
After 24 h the cell medium was replaced with differentiation medium consisting of DMEM F12 medium (Gibco) supplemented with 2 mM glutamine (Gibco), 100 U penicillin/mL, 100 U streptomycin/mL (Gibco) and N2 supplement (1 100) (ThermoFischer Scientific).
