The improved medium consisted of filtered conditioned medium obtained from all cell types and mixed with fresh medium.
A Dynomill ball mill was used and the grinding medium consisted of zirconium oxide beads.
The ball mill used was a Dynomill and the grinding medium consisted of zirconium oxide beads.
The filter medium consisted of a nonwoven geotextile with a thickness of 5 mm.
The granular medium consisted of immersed stones giving a sediment bed thickness of 10 cm.
Adipogenic medium consisted of control medium supplemented with 0.5 mM 3-isobutyl-1-methyl-xanthine, 1 μM dexamethasone, 5 μg/mL insulin, and 50 μM indomethacin (Sigma-Aldrich, St . Louis MO).
The differentiation medium consisted of Iscove's modified Dulbecco's medium/Ham's F12 1:1 (Invitrogen), 1% chemically defined lipid concentrate (Invitrogen), 450 mM monothioglycerol (Sigma), and 5 mg/ml bovine serum albumin (97% purified through crystallization; Sigma).
Osteogenic medium consisted of α-MEM supplemented with 10% FBS, penicillin (100 U/mL), streptomycin (100 μg/mL), 10 mM β-glycerophosphate, 0.05 mM L-ascorbic acid-2-phosphate, and 100 nM dexamethasone (Sigma-Aldrich, St . Louis MO).
A medium consisted of waste potato mash, malt extract and FeSO4·7H2O were defined as the optimum medium for co-culture of A. niger and S. cerevisiae SSF.
The first medium consisted of crushed brick, scoria, coir fibre and composted organics while the second comprised scoria, composted pine bark and hydro-cell flakes.
The filter medium consisted of glass beads that were either uniformly sized or a mixture of sizes with a distribution corresponding to wetland filter media.
