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Moreover, we have mapped all the pathways to our high and medium confident network.
Three of them were also predicted in our low confident PPI network, but only one (At1g03090) was detected in the medium confident network.
In our medium confident network, the protein At5g50850, which is annotated as pyruvate dehydrogenase, has an interaction with At1g54220, which is annotated as putative dihydrolipoamide S-acetyltransferase.
We collected all the components in the Glycolysis/Gluconeogenesis pathway (ath00010) from KEGG and used their neighbors in our high and medium confident network to build an ath00010 related PPI network.
We collected the proteins in fatty acid biosynthesis and leucine degradation pathways and searched their first level neighbors in our high and medium confident network to extract the PPI subnetwork out.
Similar(54)
In order to verify the interactions in our network, the orthologs in E.coliK12, S.pombe, and S. cerevisae inferred from the Arabidopsis proteins in our high and medium confident networks are extracted from the Inparanoid database.
According to the annotation information in TAIR7, which was the latest edition of TAIR annotation information when we constructed the whole PPI network, there were 1,260 function unknown proteins in our high and medium confident PPI network.
The simulated annealing algorithm[ 32] was used to annotate the function-unknown proteins based on the concept of guilt-association in both high and medium confident PPI network.
Secondly, As the interaction predictions based on ortholog are more reliable, among the LR generated from 4 different ortholog interactomes, Caenorhabditis elegance has the lowest LR with the score 92, we used 92 as the cutoff for the medium confident interactions in our network, the 16429 interactive pairs with 5363 proteins.
Considering the coverage of medium confident interactome, we suggest researchers, who are interested our dataset, to pay more attention to our medium confident PPI network.
In particular, a high confident network of mouse NSCs (NSC-GN2) was predicted.
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