Z. tritici synthetic complete (ZTSC), a defined rich growth medium, comprising 6.9 g/l yeast nitrogen base without amino acids (ForMedium, UK), 0.79 g/l complete supplement mix (ForMedium), 20 g/l glucose, 20 g/l bacteriological agar (Lab, UK).
Cells were then inoculated into 40 mL flask-fermentation medium comprising 2 g/L yeast extract, 1 g/L MgSO4·7H2O, 24 g/L (NH4 2SO4, 1 g/L KH2PO4, 0.01 g/L FeSO4·7H2O, 0.082 g/L MnSO4·7H2O, 20 g/L PIPES, and 10 g /L sodium oleate in Erlenmeyer flasks (500 mL) at an initial OD600 of 0.25.
Well-fed larvae were reared on apple juice plates with standard medium comprising of 80 g/l maize, 18 g/l dried yeast, 10 g/l soya flour 80 g/l malt extract, 40 g/l molasses, 8 g/l agar, 6.6 ml acid mix (comprising 50% propionic acid and 3.2% phosphoric acid)/l throughout larval life.
For preparation of inocula for mouse challenge, a yeast culture was grown for 18 h in 5 ml volumes of NGY medium, comprising 0.1% Neopeptone (BD-Difco, Oxford, UK), 0.4% glucose and 0.1% yeast extract (BD-Difco), incubated at 30°C with continuous rotation at 20 rpm and an angle of 5° from the horizontal.
The medium comprised of (g/l) soluble starch 47.0, Yeast extract 3.0, peanut meal 22.0, (NaCl2.7O4 2.7, NaCaCo37, CaCo3, pH 6.8 was sterilized at 121 °C, 15 lbs for 15 min.
Each liter of fermentation medium comprised 1 g KCl, 2.15 g KH2PO4·2H2O, 4 g (NH4 2SO4, 0.4 g MgSO4·7H2O, 2 g yeast extract, and 0.4 mL trace element solution.
The ATCC 2107 medium comprised the following (per liter of deionized water): 10 g peptone, 10 g beef extract, 3 g yeast extract, 5 g glucose, 5 g NaCl, 3 g sodium-acetate, 1 mL azure (0.025%), 0.5 g Cys-HCl, pH 6.8.
The freezing medium comprised 90% primary culture medium (20% FBS) and 10% dimethyl sulfoxide (DMSO).
2i/LIF medium comprises N2B27 basal medium (Stem Cells Inc).
The medium is comprised of glucose, yeast extract, ammonium chloride, and peptone in grouping of 10, 1.0, 0.5, and 1.0 g/l, respectively.
