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CGR8-MHCα-IRES::GFP [30] and Afp::GFP ES cells were maintained in ES cell growth medium comprising high glucose Dulbecco's Modified Eagles Medium (DMEM) (Mediatech) supplemented with 10% ES qualified Fetal Bovine Serum (GIBCO), non-essential amino acids (Mediatech), L-glutamine, sodium pyruvate (Mediatech), LIF (Millipore), Penicillin/Streptomycin (Mediatech) and β-mercaptoethanol (Sigma).
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To evaluate the oestrogen-stimulated growth of these cells, they were released from monolayer using 0.25% trypsin/0.53 m M EDTA and suspended in oestrogen-free medium comprised of DMEM (high glucose, phenol red-free), supplemented with 10% charcoal-stripped bovine calf serum, 2 m M glutamine, 100 IU ml−1 penicillin, and 100 μg ml−1 streptomycin.
The bacterium was isolated from rice paddy field on Biebl and Pfennig medium [ 50] and has been reported for higher yield of H2 following photofermentation in a medium comprised mainly of 2% sugarcane bagasse [ 51].
The freezing medium comprised 90% primary culture medium (20% FBS) and 10% dimethyl sulfoxide (DMSO).
2i/LIF medium comprises N2B27 basal medium (Stem Cells Inc).
High income was above the upper quartile of the income distribution, low income below the lower quartile and medium income comprised the values in between.
Serum panels contained approximately 10 test samples (sera or plasma), comprising low-, medium-, and high-titer antibody levels.
Similar to the medium and high risk interventions, the historical control comprised two phases.
Thus, the collection comprised B. napus pools with low, medium and high erucic acid contents in seeds.
The NHSII cohort comprises predominantly Caucasian women living in neighborhoods of medium to high SES.
It comprises nine questions which are then used to split patients into low, medium and high risk of poor outcome.
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