The localized deformation is described by considering a structured medium comprising the intact masonry intercepted by a distinct macrocrack.
The vitrification medium comprised a standard buffer for fish spermatozoa (Cortland®medium) + 10% DMSO + 2% BSA + 0.13 M sucrose + SP at concentrations of 30% (group 1), 40% (group 2) or 50% (group 3).
The freezing medium comprised 90% primary culture medium (20% FBS) and 10% dimethyl sulfoxide (DMSO).
The differentiation medium comprised serum-free DMEM with 10 mg/ml insulin and 10 mg/ml transferring.
The culture medium comprised 1 1 Dulbecco's modified Eagle's medium (DMEM) + Ham's F-12 with penicillin and streptomycin (all from Invitrogen Corporation, Carlsbad, CA, USA).
The culture medium comprised of Dulbecco's modified Eagle's medium (GIBCO®; Life Technologies) containing 15% Knockout™ Serum Replacement (Life technologies), GlutaMAX™ (Life technologies), non-essential amino acids, and β-mercaptoethanol.
The transport medium comprised Hanks balanced salt solution supplemented with 10% glycerol, 200 U/mL penicillin, 200 μg/mL streptomycin, 100 U/mL polymyxin B sulfate, 250 μg/mL gentamicin, and 50 U/mL nystatin.
The adipogenic differentiation medium comprised DMEM-DG medium containing 1 μmol/L dexamethasone, 0.5 mmol/L 3-isobutyl-1-methylxanthine (IBMX), 10 mg/L insulin, and 100 mmol/L indomethacin.
Each country will deliver either 1) the original client record files or 2) a data storage medium comprising the scanned files to the RTPC coordinating centre by secured post or express courier or even personally by a researcher.
The Congo red agar medium comprised BHI (37 g/L), sucrose (50 g/L), No. 1 agar (10 g/L) and Congo Red stain (0.8 g/L).
