Sentence examples for medium comprised of from inspiring English sources

After incubation, 1,000-fold dilutions were prepared and 100 μL of the diluted samples was plated on a solid medium comprised of TSB.

The loosely adherent RPE cell layer was gently peeled off each eyecup using fine forceps, transferred to a 15 ml tube containing culture medium comprised of Medium199, 15% FBS (Gibco, Carlsbad, CA) and 1x MEM with non-essential amino acids (Sigma, St . Louis, and then triturated with a transfer pipette to form a dissociated cell suspension.

The cells were cultured in a specialized growth medium comprised of a triple-sugar minimal essential medium α L-15 base supplemented with 10% fetal bovine serum, designated as GTSF-2 [85], and incubated at 37°C at 5% CO2 environment.

Media was replaced with hESC differentiation medium, comprised of Glasgow Minimum Essential Medium(GMEM) supplemented with 10% knockout serum replacement, 0.1 mM nonessential amino acids (Invitrogen), 1 mM sodium pyruvate (Sigma-Aldrich, St . Louis U. S. A ., and 0.1 mM β-mercaptoethanol (Millipore).

The culture medium comprised of Dulbecco's modified Eagle's medium (GIBCO®; Life Technologies) containing 15% Knockout™ Serum Replacement (Life technologies), GlutaMAX™ (Life technologies), non-essential amino acids, and β-mercaptoethanol.

To evaluate the oestrogen-stimulated growth of these cells, they were released from monolayer using 0.25% trypsin/0.53 m M EDTA and suspended in oestrogen-free medium comprised of DMEM (high glucose, phenol red-free), supplemented with 10% charcoal-stripped bovine calf serum, 2 m M glutamine, 100 IU ml−1 penicillin, and 100  μg ml−1 streptomycin.

The medium comprises of DMEM/F12 with GlutaMax, BSA 1.8%, β-Mercaptoethanol 0.1 mM, 1× StemPro protein cocktail (All from Invitrogen) and 8 ng/ml of basic fibroblast growth factor (FGF2, Sigma).

Following 1.5 h incubation in a shaking water bath at 37°C, the cell suspension was filtered through a 40 µm mesh (Fisher Scientific, Loughborough, UK) to remove undigested material and the filtrate was resuspended in washing medium, comprising of HBSS with 10% fetal bovine serum (FBS, Sigma).

For experiments, 90% confluent cells 2 to 4 days after collection were disassociated using Accutase (Sigma), resuspended in complete medium, comprising of DMEM (without phenol red)/Hams F-12 nutrient mixed 1∶1 (Sigma), supplemented with 10% FBS, 20 mM Hepes, 100 µg/ml streptomycin, 100 U/ml penicillin, and 2 mM l-glutamine (all Sigma).

The bacterium was isolated from rice paddy field on Biebl and Pfennig medium [ 50] and has been reported for higher yield of H2 following photofermentation in a medium comprised mainly of 2% sugarcane bagasse [ 51].

Here, T. vincentii and T. medium comprised only ca. 5% of the total number of clones obtained for oral phylogroup 1 treponemes; the rest corresponding to yet-to-be cultivated OTUs.