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When combined with the developed regeneration procedure [1] we show that novel medium compositions and optimised culture steps offer a complete way leading from canes to embryogenic callus initiation and to subsequent plant regeneration.
There is usually a relationship between the medium compositions and secondary metabolites (Azma et al. 2011; Elibol 2004).
Any microbial fermentation process can be affected by medium compositions and process parameters, and therefore statistical experimental design methods were used efficiently to optimize fermentation medium of strain ZX01.
In the present study Plackett Burman design and Box Behnken design of the RSM were used to optimize the medium compositions and cultivation conditions for the highest l-DOPA production by using Y. lipolytica-NCIM 3450.
Cultivations in shake flasks were conducted to investigate different medium compositions and to analyze the Surfactin production of different Bacillus strains in medium B and medium C (Table 1).
In 8 cases the conflicting outcomes could be sorted out with literature-based assessment of the in vitro medium compositions and restriction of indicated inbound fluxes (conditional essentiality).
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Eleven factors, medium composition and some cultural conditions, were screened.
The medium composition and the reaction conditions were then simultaneously optimized.
Also, cellular environment, medium composition and dynamic culture system influenced liver-specific functions of hepatocytes beside ECM.
Our results showed that the performances were closely related to medium composition and rare codon percentages of raw DNA sequences.
Effect of medium composition and culture conditions on agarase production by Agarivorans albus YKW-34 was investigated in shake flasks.
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