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The section holds also the process condition, medium composition, protocol description and a direct link to the Excel data file for download.
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A simple and an efficient in vitro propagation protocol of Gentiana scabra Bunge by optimizing the medium composition and ventilation closure treatments has been developed.
In the present study, we have developed a simple and an efficient in vitro propagation protocol of Gentiana scabra Bunge by optimizing the medium composition and ventilation closure treatments.
It was apparent from a comparison of protocols used in our laboratories and those of others that culture medium composition represented a variable in different laboratories.
We describe the experimental design protocol used to achieve the culture conditions necessary to obtain both high-cell-density and TAG accumulation; specifically, we describe the importance of the C/N ratio of the medium composition.
Experimental designs were practiced for optimisation of the medium composition.
First, the medium composition was optimized in shake flask cultures.
Eleven factors, medium composition and some cultural conditions, were screened.
Gudiña, E., Teixeira, J. & Rodrigues, L. Biosurfactant-producing Lactobacilli: screening, production profiles, and effect of medium composition.
To explore the extent to which the medium composition affects the accuracy of FBA predictions, we repeated all simulations using Ham's medium, a nutrient poor medium adopted in previous studies to predict in silico gene essentiality of cancer cells33,39.
Table 1 Materials used and treatment protocol Material Composition Protocol Manufacturer Adper Scotchbond Multiuse adhesive system Primer: HEMA, polyalkenoic acid.
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