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Response surface methodology was applied to determine the optimal medium composition for sulphuric acid production by an indigenous Thiobacillus thiooxidans.
The medium composition for bio-butanol production was optimized using the Box Behnken design and response surface methodology (RS M.
The medium composition for bacteriocin production by Lactococcus lactis ATCC 11454 was optimized using response surface methodology.
The optimal medium composition for maximum glucan production was sucrose 5.95%, peptone 0.52% and yeast extract 2.9%.
The base liquid medium composition for MP was almost the same as that for MC, except that butyrate solution was not added.
Medium composition for each ventilation closure treatment consisted of half strength MSBM supplemented with 0.1 mg/L−1 NAA, 3% sucrose and 0.3% gelrite.
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Therefore, two medium compositions for enhancing biomass and surfactin specific production were proposed and tested in continuous regime in a bubbleless membrane bioreactor.
In this study, response surface methodology was employed to optimize the medium compositions for the production of exopolysaccharides (EPS) from endophytic bacterium Paenibacillus polymyxa EJS-3.
As shown in Fig. 1, 2 and 3, the optimal values of medium compositions for obtaining the maximum yield of GP-1 lie in the following ranges: soluble starch 12 14 g/L, yeast extract 3.67 4.33 g/L and inorganic salts 3.37 4.03 g/L.
For successful one-pot process for ethanol production, medium composition, times for inoculum of each microorganism and substrate addition should be carefully considered.
The following defined medium composition optimized for ethanol production from high xylose concentration feeds [ 10] was used in all precultures and growth cultures for inhibitor tolerance bioassays: purines/pyrimidines - 10 mg/L adenine, cytosine, guanine, uracil, and thymine; macro-minerals - 1 g/L K2HPO4, 1 g/L KH2PO4, and 0.75 g/L MgSO4.
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