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Optimal medium components were determined using fractional factorial design.
The concentrations of five fermentation medium components were optimized.
Solvents and culture medium components were from Nacalai Tesque Inc (Kyoto, Japan).
Since the bioreactor cultivation was realized as a batch cultivation, no further medium components were added.
Eleven medium components were examined for their significance on enzyme production using Plackett Burman factorial design.
The following medium components were analyzed apart from urea in optimization experiments: MgCO3; K2HPO4; MgSO4; Cand2 aNaClaCl.
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The optimization of medium components was performed using both Plackett Burman design and response surface methodology.
The effect of 12 medium components was studied in 16 experimental trials.
Bond session in light components and chelation after oil oxidation in medium components are responsible for this variation.
The predicted cellulase production obtained from the model using the above optimum concentration of medium components was 29.95 U/mL.
Optimization of fermentation process and the medium components are reported as key strategies for economic recovery of pigments.
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