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Due to the evaporation the medium components became more concentrated and, therefore, the signals (especially the scattered light signal) increased.
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Increasing the gain level to medium (middle row), the even harmonic components become more suppressed in the real AC30 response, while the overall spectral envelope gets brighter.
In addition, large components become sparser as the threshold becomes more stringent.
In 2011 the dominant component became age.
Optimal medium components were determined using fractional factorial design.
The concentrations of five fermentation medium components were optimized.
Solvents and culture medium components were from Nacalai Tesque Inc (Kyoto, Japan).
Medium components were from Difco Laboratories (Detroit, MI).
All medium components, except LIF, were from Invitrogen.
All medium components were heat-sterilized (121°C, 20 min).
Cell culture medium components were purchased from Biochrom, Berlin, Germany.
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