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Subsequently, optimum concentration of each medium component was determined using the orthogonal matrix method.
The contribution of the incompletely bleached medium component cannot be removed totally by an early background subtraction approach.
Primarily, significance of each medium component with respect to CoQ10 production was identified by Plackett Burman design.
Nitrogen (N) sources, the critical medium component, were optimized for squalene production by microalga Aurantiochytrium sp. in heterotrophic cultures.
In order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization.
A mathematical model was developed to show the effects of each medium component and their combinatorial interactions on glucan production.
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Optimal medium components were determined using fractional factorial design.
The concentrations of five fermentation medium components were optimized.
The study involves step wise optimization protocol incorporating screening of medium components.
Plackett-Burman statistical experimental design was applied to evaluate the fermentation medium components.
Firstly, sucrose, yeast extract, peptone and Mg2+ were identified to be key medium components by PBD.
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