Sentence examples for medium complemented with from inspiring English sources

Briefly, 50 µl of each last bacterial suspension in 10 mM MgSO4 (see Bacterial strain and growth media) was inoculated in 96-well plates containing 1 mL 1/10 diluted 869-rich medium complemented with 50 mg mL−1 tryptophan and incubated at 30 °C for 4 days at 150 rpm in the dark.

Cultures were maintained in neurobasal medium complemented with B27 and glutamate (Invitrogen) as described elsewhere [52].

Five microliters drops were then plated on SD or SG medium complemented with appropriated amino acids.

ES34+ and ES34− were tested for their ability to proliferate clonally in methylcellulose-based medium complemented with hematopoietic growth factors.

SKBr3 cells were cultured in McCoy's 5A medium complemented with 10% fetal calf serum (FCS), 100 units/ml penicillin and 100 µg/ml streptomycin.

The colon carcinoma cell line HCT116 and the ORC2-hypomorhic cell line e83 were maintained in McCoy's medium complemented with 10% FCS [36].

The cells were grown in DMEM medium (Dulbecco's modified Eagle's medium; ICN Flow Laboratoriess) complemented with 10% heated inactivated fetal calf serum (FCS), 30 units/ml of penicillin, 30 μg/ml of streptomycin and 2 mM of L-glutamine.

The cells were cultured until near confluence, and the medium was replaced by 400 μL of reaction medium (RPMI complemented with 2 mg/mL BSA, 4.8 mg/mL HEPES, 1 U/mL penicillin G and 1 μg/mL streptomycin).

Only for the plates used for germination, this basic medium was complemented with 5 g L−1 sucrose.

Cells were cultivated in keratinocytes serum free medium (KSFM) complemented with 0.25% human recombinant EGF (Epidermal Growth Factor), 2.5% pituitary bovine extract, and 5% fetal bovine serum (FBS).

Mouse embryonic fibroblast (MEF) and HeLa cells were cultured in Dulbecco's modified Eagle's medium (DMEM), complemented with 10% fetal calf serum (FCS), 100 units/ml penicillin, 100 µg/ml streptomycin.