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The decreased PINP level observed for cells cultured on 3D PLA/HA composite woven scaffolds in OS medium compared to the 3D PLA woven scaffold suggests that the cells cultured on PLA/HA composite scaffolds had already reached their PINP secretion peak by this time point and had differentiated into osteoblast cells.
The passive film on stainless steel, modified in this way, proved to be more stable to the effect of aggressive sulfuric acid medium, compared to the case of natural passive film.
This means that the environmental impact and risk of failure are considered as medium compared to the other options.
In the mls1Δ strain (SCKK009), less acetate was accumulated in the medium compared to the cit2Δ strain and acetate was slowly consumed until the end of fermentation.
Among seven additives, only glucose significantly promoted the decrease in the pH value of the culture medium compared to the control (Fig. 4b).
lactis SLPE1-3, it delayed the decay of SLPE1-3 in the culture medium compared to the control without the additive riboflavin.
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As expected, similar to the results of MTS assay, the SPIO nanoclusters at all concentration examined did not increase the release of LDH in the culture medium compared to that of the unlabeled cells (Fig. 5).
The quantification of GAGs indicated that GAG synthesis was significantly higher in the TEC exposed to the chondrogenic medium compared to those generated in the absence of such components (Fig. 2b).
With the thresholds used (false discovery rate (FDR) < 0.01, log2fold-change > 0.4), 113 ORFs were found to be up-regulated (Additional file 1) and 121 ORFs were down-regulated (Additional file 2) when HMF and furfural were present in the feed-medium, compared to the control.
Isocitrate dehydrogenase, which is one of the TCA enzymes bypassed during the glyoxylate pathway, was decreased significantly in acetate medium compared to TSB, suggesting that the glyoxylate pathway is favored over the TCA cycle during growth on acetate.
The yield of wet cells was ∼10 g L 1 from the synthetic Fe medium (compared to ∼35 g L 1 from the standard medium).
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