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Ioxitalamate (Telebrix 300) is an ionic iodinated contrast medium commonly used for discography or percutaneous endoscopic lumbar discectomy (PELD), though it has side effects such as anaphylactic shock and renal toxicity.
Due to strong aggregation tendency of AuNPs in plant growth media (MS medium), commonly used for plant growth in vitro [23], we had to optimize mutual ratio of these two components to avoid NPs aggregation while preserving acceptable conditions for plant growth.
The film's skateboarding sequences were shot on Super 8 mm film, a medium commonly used in skate videos, and the remainder of the film was shot on 35 mm film, Van Sant's medium of choice.
Cultures of 80 L volume (10cm depth) were established for each strain using a culture medium commonly used in the productive process of Pigmentos Naturales S.A. (Patent 43154, 2007).
For electroporation, we used 20 μl total volume to accommodate the CRISPR/Cas reagents (Cas9 mRNA, sgRNA, donor oligonucleotide) reconstituted in TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 7.5) and embryos suspended in Opti-MEM, a medium commonly used in transfection experiments.
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To select a suitable medium for the growth and lipid accumulation of Chlamydomonas sp. JSC4 after pre-culturing on Modified Bold 3 N medium, five commonly used media (basal medium 1/3 N, Modified Bristol 1/2 N medium, BG11 1/4 N medium, Modified Bold 3N medium, and high salt 1/2 N medium) were used to grow the JSC4 strain for lipid accumulation.
Two different methodologies to dissolve the examined compounds, using either organic solvents and water, or dimethyl sulfoxide (DMSO) and buffered medium, are commonly used in these bioassays.
Although this experimental condition is far from in vivo situation, transfections carried out in serum-complete medium are commonly used to check serum resistance of lipoplexes prior to performing animal studies [16], [31].
Fermentations were carried out at 24°C, using the culture medium MS300, commonly used as synthetic grape must [ 93].
First, this study used a single dose of MPH (0.5 mg/kg/day), which is in the low to medium range commonly used in clinical practice.
Tare et al. [ 15] reported that chondrogenic medium (CM), commonly used for chondrogenesis of MSCs and other stem cells, was effective for chondrocyte differentiation of ATDC5.
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