Sentence examples for medium changes with from inspiring English sources

After 3 5 more days of daily medium changes with NuFF-1 MITC-conditioned Pluriton medium, visible colonies were counted on AP-stained and unstained wells.

Partial medium changes with fresh CM were performed every 3 days.

The pellet cultures were maintained under these conditions for 5, 7, and 10 days with medium changes with and without PPS every 2 to 3 days.

Subsequently, ESNs underwent full medium changes with B27 on DIV 4, 8 and 12. On DIV 8 the media was supplemented with 30 µM 5-fluoro-2'-deoxyuridine and 70 µM Uridine (Sigma-Aldrich) to select against any remaining glia.

At this stage we have decided to investigate the contributions of the overexpressed AhR on the expression of several diagnostic markers under both fresh serum (3 hrs after the medium change with fresh serum) and serum starved (72 hrs after the last medium change with serum).

The siRNA/survivin or siRNA/control was transfected with 50 n M siRNA duplexes using the HVJ Envelope VECTOR KIT (Ishihara Sangyo, Osaka, Japan) according to the manufacturer's instructions, and incubated for 4 h followed by medium change with fresh MEM.

Cultures were subject to half-medium changes with fresh cytokines and conditioned medium added every 48 h.

With the medium changes the way we interact with text, the ways we read and write, and extract information.

24 h later, the medium was switched to CDF12 medium with medium changes every day.

hMSCs were expanded until confluent, with medium changes every 3 4 days.

The cells were cultured in this supplemented medium for 2 wk with medium changes every 2 3 d (30).