Sentence examples for medium change with from inspiring English sources

At this stage we have decided to investigate the contributions of the overexpressed AhR on the expression of several diagnostic markers under both fresh serum (3 hrs after the medium change with fresh serum) and serum starved (72 hrs after the last medium change with serum).

The siRNA/survivin or siRNA/control was transfected with 50 n M siRNA duplexes using the HVJ Envelope VECTOR KIT (Ishihara Sangyo, Osaka, Japan) according to the manufacturer's instructions, and incubated for 4 h followed by medium change with fresh MEM.

After 3 5 more days of daily medium changes with NuFF-1 MITC-conditioned Pluriton medium, visible colonies were counted on AP-stained and unstained wells.

Partial medium changes with fresh CM were performed every 3 days.

The pellet cultures were maintained under these conditions for 5, 7, and 10 days with medium changes with and without PPS every 2 to 3 days.

Subsequently, ESNs underwent full medium changes with B27 on DIV 4, 8 and 12. On DIV 8 the media was supplemented with 30 µM 5-fluoro-2'-deoxyuridine and 70 µM Uridine (Sigma-Aldrich) to select against any remaining glia.

Cultures were subject to half-medium changes with fresh cytokines and conditioned medium added every 48 h.

Fresh AZA was added with every medium change and supplemented with 300 nM Trichostatin A (Sigma-Aldrich) for the last 16 h of culture.

We observed that 24 h after medium change, cells treated with control medium underwent apoptosis, while cells treated with G3 containing medium grew normally.

A daily full medium change was performed with warm STEMdiffTM Neural Induction Medium.

Cells were then washed with medium for three times and maintained in PMM medium with medium change every 2 3 days.