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Indeed, the cell numbers recorded prior to each medium change were only slightly reduced for the treated cells as compared with the untreated ones (not shown).
Discard of non-adherent cells and first medium change were performed after 2 days: thereafter medium was changed three times a week.
The time series bioluminescent data of triplicate samples, which were measured from 0.5 to 3.8 days after the medium change, were fitted to a cosine curve using R version 2.9.1.
When viewed collectively these morphological responses to medium change were limited to particular morphological features.
Data beyond 192 hr before medium change were also trimmed except for one group (1 Fx/Fx control and 1 BKO), which was affected by an electrical shutdown on day 6.
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A daily full medium change was performed with warm STEMdiffTM Neural Induction Medium.
The medium change was repeated at 24 h and at every 48 h afterwards.
On the first chip, culture medium change was performed on day 2, and fixation and labeling on day 3.
The first medium change was after 7 days.
Complete medium change was performed on day 6 and 12.
Medium change was done 24 h before treatment.
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