Sentence examples for medium change to from inspiring English sources

The first medium change to remove debris from attached cells occurred 1 h and 24 h after plating, if the cells grew on Transwells or 24 h after plating, if the cells grew on cell culture flasks or plates.

After 4 days in suspension culture, aggregates were counted and plated en bloc onto laminin-coated coverslips, being then cultured for 4 days in RHB-A medium (with an intermediate medium change) to allow differentiation.

Four days after virus infection, hESCs were treated with either G418 (400 µg/ml) or Blasticidin (5 µg/ml) for 36 hours, rinsed twice with DPBS to remove drugs, and cultured for two to three days with daily medium change to permit recovery.

siRNA-B sense: 5'-GGAGCAGGAAAACCGGAAGtt-3'; antisense: 5'-CUUCCGGUUUUCCUGCUCCtt-3) (Ambion, Austin-TX, USA) were added singularly in 50 µL serum-free Optimem (Invitrogen, Carlsbad-CA) at 100 nM final concentration using as vehicle SilentFect liposome formulation (BioRad Laboratories, Hercules-CA) for 24 96 h, without any other medium change, to achieve DJ-1 downregulation.

Condition of serum starvation as experimental setting was induced by medium change to medium without supplements 24 h after seeding.

Following a medium change to remove secNluc activity resulting from basal expression, cells were treated with FSK to observe both the kinetics of signal accumulation and a dose response (Supplementary Figure s13).

Drugs were administered as complete medium changes to fresh culture medium containing the drug but otherwise identical to control medium.

The cells were incubated for 24 h and the medium changed to 100 µl fresh medium with and without 0.5 µM PPP.

The cells (105 cells per 24 well) were cultured overnight before addition of tick extract, and the medium changed to RPMI 1640 supplemented with 2.5% FCS and antibiotics.

Then cells were washed and the medium changed to Claycomb medium 10% FBS.

So, Opn4m requires LD cycles plus medium changes to display a temporal oscillation as shown here).