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This medium change resulted in enhanced biofilm formation of this clinical isolate on the un-patterned control surfaces, whereas the micro-patterned surfaces maintained an inhibitory effect, with a 58% reduction compared to controls (p = 0.009) (Figure 4).
Failure to passage the cells in a timely manner (despite medium change) resulted in massive cell death, suggesting that higher cell densities negatively affected stem cell survival even in the presence of trophic support.
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An earlier or delayed medium change results in a partial restoration of the colony formation or in smaller colonies.
The rhythms gradually declined in amplitude over several days in culture, but amplitude could be restored by a medium change (results not shown), as reported previously for other brain regions (Abe et al., 2002).
No changes resulted.
Following a medium change to remove secNluc activity resulting from basal expression, cells were treated with FSK to observe both the kinetics of signal accumulation and a dose response (Supplementary Figure s13).
The theoretical protein structure prediction method homology (or comparative) modeling [25] [27] relies on the observation that the structural conformation of a protein is more highly conserved than its amino acid sequence, and that small or medium changes in sequence typically result in only small changes in the 3D structure [28].
Before heat shocking (see Results S1), cells were left in transfection condition for 48 hours followed by medium change.
This results in an accumulation of unprocessed procollagen in the cell culture medium [ 73] where it does not belong and is discarded with every medium change.
Drug was renewed at each medium change.
By pH variations of medium, changes occur in its ionic form, resulting in change in the enzymes activity because of the reduction of its specificity.
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