Sentence examples for medium change lasted from inspiring English sources
A medium change lasted a couple minutes and cultures were allowed to equilibrate for an additional 30 minutes prior to beginning stimulation.
Twenty-four hours after the last medium change, 110 µl of a solution containing 10 µl of MTT (Sigma) solution (5 mg/ml) and 100 µl of medium were added and the cultures incubated for a further 4 hours.
To assess hepatocyte function, culture medium was collected 24 hr after the last medium change.
Culture medium was changed every 3-4 dand and culture supernatant was collected was collected 24h after the last medium change.
Culture medium was changed every 2-3 dand and culture supernatant was collected 24h after the last medium change.
Samples were further clusterized by the time in culture after the last medium change (3 or 60 hs) and finally separated as a function of TNF treatment.
In either case, T17b EPC culture supernatant was collected at day 4 24 hrs after the last medium change and VEGF secretion was quantified using a VEGF ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions.
At this stage we have decided to investigate the contributions of the overexpressed AhR on the expression of several diagnostic markers under both fresh serum (3 hrs after the medium change with fresh serum) and serum starved (72 hrs after the last medium change with serum).
Fresh AZA was added with every medium change and supplemented with 300 nM Trichostatin A (Sigma-Aldrich) for the last 16 h of culture.
The medium changes but the craft continues.
