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Plating efficiency was assessed on the second day after the first medium change.
The culture was kept for another two weeks with a medium change every 3 days.
Blastocyst formation was suppressed in cultures with no medium change (P = 0.004; decreased to approximately one-fourth of that with medium change).
How will the change in medium change your perception of the subject?
In the default 'spontaneous' protocol, basal cells were continuously cultured for 3 weeks in WIT post confluence without medium change.
In the default 'spontaneous' protocol, basal cells were continuously cultured for 3 weeks post confluence without medium change.
After the first medium change, images of adherent colonies were acquired over five different areas within each well.
A daily full medium change was performed with warm STEMdiffTM Neural Induction Medium.
From day 5, cells were maintained in RPMI/B27 with medium change every 2 3 days.
After medium change, most neural stem cells had the appearance of typical astrocytes.
Pellets were cultured for 21 days with a medium change every other day.
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