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Although plant oils tend to be largely unsaturated, there are notable exceptions, such as coconut fat, which is highly saturated but nevertheless semiliquid at room temperature because its fatty acids are of medium chain length (8 to 14 carbons long).
For example, medium chain length fatty acids are extensively used for the production of soap and detergents (Dyer et al. 2008), and medium chain length alkanes are main components of jet fuel (Kallio et al. 2014).
A strategy aimed at the production of medium chain length fatty alcohols in E. coli was published recently (Youngquist et al. 2013).
Coconut oil is composed of medium chain length saturated fatty acids that improve the solubility of carotenoids inside the body of the fish [16].
This substrate specificity assay was repeated with purified AAE15 (Fig. 2b), and the result confirmed the specificity for medium chain length fatty acids.
Engineered microbial biosynthesis of medium chain length alkanes, which constitute the major fraction of petroleum-based jet fuels, was recently demonstrated.
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A novel strain of Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize medium chain-length polyhydroxyalkanoates (mcl-PHAs).
Short chain-length monomers generally confer toughness and high crystallinity, and medium chain-length monomers generally confer elasticity and low crystallinity.
These PHAs are typically classified as short chain-length (SCL) PHAs, which contain repeating units of 3 5 carbons, and medium chain-length (MCL) PHAs containing 6 14 carbons.
This enables E. coli to grow aerobically on fatty acids with medium chain-length [ 214].
The affinity of FadD for medium chain-length fatty acids can be enhanced by directed mutagenesis [ 125].
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