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Schnyder corneal dystrophy and normal human keratocytes were cultured at 37°C in Fibroblast Medium (catalogue no. 2301) also obtained from ScienCell Research Lab.
Cell culture reagents were purchased from Gibco, USA; RPMI 1640 medium; catalogue number (A10491-01), Dulbecco's Modified Eagle Medium; Catalogue number (31100 035) were obtained from GIBCO, UK.
DMEM (Dulbecco's modified Eagle's medium; catalogue number 12320-032), penicillin, streptomycin, Amphotericin B and trypsin were obtained from Invitrogen and FBS (fetal bovine serum) was from Hyclone.
After several washes in PBS, the embryos and larvae were mounted in Vectashield Mounting Medium (catalogue no. H-1000, Vector Laboratories Inc., Burlingame, CA, USA) and analysed with a confocal microscope.
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After being washed in PBS, the sections were mounted in 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) containing fluorescence mounting medium (Vectashield, catalogue no. H-1200, Vector Laboratories Inc., Burlingame, CA, USA).
The second 5 mL of blood was inoculated into an in-house bottle containing 40 mL of broth, which consisted of 37 g of brain heart infusion medium broth (catalogue no, 211059; Becton Dickinson, Franklin Lakes, NJ) and 0.25 g of sodium polyanatholesulfonate (catalog no.1000907362; Sigma, St . Louis MO), in 1 liter of purified water.
The isolates were transported to the Shoklo Malaria Research Unit (SMRU) field laboratory within 6 h of collection; the stages of parasitemia were assessed, and samples were then depleted of white blood cells (WBCs) by cellulose medium fiber (Sigma catalogue no.
Microcystis sp. RST 9501 was grown in Z8 medium replaced with stable isotope S-labeled MgSO4.7H2O (catalogue no. IS7080; 90 atom % S; Icon) for the detection and identification of sulfur-containing microcystins.
The fish were mounted in 0.2% agarose in E2 medium (Ultra-Low gel temperature Type IX Agarose, SIGMA catalogue number A5030) on a drop of 0.3% methyl cellulose in E2 medium between bridged cover slips [47].
For most observations we vitally stained mineralized bone in E2 embryo medium containing 50 µg/ml Alizarin Red S (JT Baker catalogue # A475-03) and 10 mM HEPES, pH 7.0.
So we need institutions doing the work to catalogue, preserve, and reflect on what these mediums mean to us.
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CEO of Professional Science Editing for Scientists @ prosciediting.com