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We used this selectivity difference to design amino acid determinations of diluted cell culture and fermentation broth media, including Bacto yeast extract peptone dextrose (yeast culture medium) broth, Luria Bertani (bacterial culture medium) broth, and minimal essential medium and serum-free protein-free hybridoma medium (mammalian cell culture media).
A capsule-like complex (CLC) was effectively extracted from the cell surface of an F. tularensis live vaccine strain (LVS) lacking O-antigen with 0.5% phenol after 10 passages in defined medium broth and growth on defined medium agar for 5 days at 32°C in 7% CO2.
Anaissie et al. [ 22] evaluated YNB, RPMI 1640 medium broth, and Eagle's minimum essential medium broth, and found that RPMI 1640 broth was a suitable medium to test the susceptibility of C. neoformans.
The bacteriostatic versus bactericidal nature of the extracts was determined by subculture from wells containing inhibitory dilutions into neat SP-4 or mT-soy medium (broth and agar) at 37°C.
Similar(56)
The MICs of nalidixic acid, levofloxacin, ampicillin, and azithromycin were determined by the MIC microbroth dilution method with an in-house Haemophilus test medium broth (5 ) and interpreted according to National Committee for Clinical Laboratory Standards (NCCLS) guidelines (6 ).
Initial fermentation of E. coli BL21 DE3:pAV01 in baffled flasks showed that high cell density was achieved when using complex media, Luria Bertani (LB and Terrificc medium broth (TB) (10 and 14 g/L wet weight, respectively), as compared to mineral media M9, modified minimal medium (MMM) and Riesenberg mineral medium (RM) (7, 8 and 7 g/L, respectively).
The respective MIC and MBC values of all 28 positive hits in standard medium (TS broth) and screening medium (20% TS broth + 80% M9 medium) are presented in Table 3A and B. The negative control (DMSO) and positive control (gentamicin) were run in parallel with a recorded MIC for gentamicin towards S. aureus at 1.2 μg/mL and MBC of 2.4 μg/mL.
To evaluate the production of HCP EHEC O157 H7 strain EDL933 was cultured in different media [Colonization factor antigen (CFA) agar, Trypticase soy broth (TSB), Luria Bertani (LB) broth, Mueller Hinton (MH), Dulbecco's minimal Eagle medium (DMEM), and Minca minimal medium broth] with shaking, at 37°C and 5% CO2.
Staphylococci and streptococci were grown in Tryptic Soy Broth (TSB; Difco Laboratories) medium with shaking at 37°C, E. coli was grown in Luria-Bertani (LB; Difco Laboratories) medium broth at 37°C with shaking, and lactobacilli were grown in de Man, Rogosa and Sharp (MRS; Difco Laboratories) medium at 37°C without shaking.
After being autoclaved, the filtered polymyxin B, kanamycin, iodoacetic acid, nalidixic acid and 2,3,5-triphenyltetrazolium chloride each was serially added and poured onto petri dishes for further uses); the selective growth medium for E. coli was Endo medium (Endo broth and Agar Bacteriological. After being autoclaved, the medium was poured onto petri dishes for further uses).
After 72 h, 100 mL of medium broth was used as an inoculum for bioreactor culture.
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