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The values of effector cells' spontaneous LDH release, target cells' spontaneous LDH release, target cells' maximum LDH release, and culture medium background reading were also measured.
The container included six spheres with a diameter of 30, 36, 36, 28, 24, and 19 mm and a specific activity of 0, 7, 3.5, 3.5, 3.5, and 3.5 times that of the surrounding medium (background), respectively.
The relative amount of released LDH was calculated as follows: % released LDH (sample) = (sample - medium background)/ total LDH - medium background) ×100.
Stimulation of SPC from PBS-treated controls indicated that neither 4YSyn nor N-4YSyn induced significant proliferation above medium background levels (Figure 8B).
EYFP concentrations above10 ng/ml were detectable over medium background (Additional file 1, Figure S1).
Inline, spectra were acquired every 2 5 min against a medium background, resulting in approximately 2000 spectra for each fermentation run.
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The medium backgrounds of absorbance and fluorescence were determined from blank wells loaded with LB media and were subtracted from the readings of sample wells.
This disturbing story, covered in the Hindi and English print media, highlights the long-standing divide between students who come from English-medium backgrounds and those who come from "vernacular" ones.
Cytotoxicity was calculated as follows: Cytotoxicity = 100× amount of LDH in basal medium minus background) / (amount of LDH of lysed apical cells minus background).
Background elimination was performed by subtraction of the Raman spectra of incubation medium and background equalization of measured spectrum and corresponding model (Supplementary Figure S3).
The cardiac phantom studies performed with attenuating medium and background activity showed that the half-scan time images processed with WBR had better contrast recovery and background uniformity than did the full-scan time FBP reconstruction.
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