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In response to this knowledge gap, we performed a transcriptomic analysis to study the changes in gene expression of Lp exposed to Fraquil, an artificial freshwater medium composed of trace amounts of salts and metals, compared to Lp grown in rich medium (AYE broth).
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The L. pneumophila strains used are derivatives of the strain Lp02 (thyA Δ(hsdR-lvh) rpsL) [48], and were grown on charcoal-yeast extract (CYE) solid medium or ACES-yeast extract (AYE) [48]; Lp03 contains the dotA3 mutation [48].
Legionella pneumophila strains were grown on buffered charcoal-yeast extract solid medium or ACES-buffered yeast extract (AYE) broth culture media (Feeley et al., 1979; Gabay and Horwitz, 1985; Berger and Isberg, 1993).
"Aye, aye.
Aye, aye, aye, Weequahic High!
Aye! Unda!
"Oh aye.
Mibbes, aye.
Yes?" "Aye.
Right, aye.
Aye, unofficially.
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