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The samples were taken out of the release medium at specific but different time intervals.
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The dissolution value (DA/N) reported used the relative change in the amount of dissolution in the alcoholic medium compared to the values in the purely aqueous medium at a specific time point.
To our knowledge Mrl2 expression achieved the highest level of a fungal laccase in P. pastoris reported so far with 281,000 U l−1, which corresponds to 1.05 g Mrl2/l medium at a specific activity of 248 U mg−1.
The fluence rate Φ in a homogeneous and infinite diffusing medium at a specific distance r from a point-like CW source [42] emitting an unit power is given by: (4).
The first pump delivers fresh medium at a specific flow rate to a second container which contains the antimicrobial agent at a concentration equivalent to its Cmax (The peak concentration of a drug observed after its administration in the human body).
The results showed that MSCs maintained in osteogenic medium, secreted factors at specific time points that induced alkaline phosphatase activity (ALP) in exogenous MSCs as well as their migration.
After culture for 24 h, the culture medium was replaced with medium containing bio-AgNPs prepared at specific concentrations (0 to 50 μg/mL) and chem-AgNPs (0 to 100 μg/mL).
Receptor medium (2 mL) was withdrawn at specific time intervals over a 24 h period and immediately replaced with fresh prewarmed buffer.
All the experiments were performed at 20 °C, with the culture medium at pH 9.0, a specific rate of air supply of 1.0 vv−1 m−1 and a continuous illumination of 68 μEm−2 s−1.
At specific times, the medium was sampled for analysis of drug concentration.
We isolated from 1 to 6 colonies from each positive milk sample and subculture them in specific broth medium at 37°C, in a 5% CO2 atmosphere for 3 days.
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